原癌基因MET启动子的G-四链体结构

摘要

目的：解析原癌基因MET启动子中的G⁃四链体结构，并初步探索其功能。方法采用圆二色光谱、紫外吸收光谱、非变性凝胶电泳和PCR Stop实验分析MET启动子富含G的Pu23WT序列的G⁃四链体拓扑结构特征，并分析该结构的功能。结果MET启动子的Pu23WT序列形成了分子内平行型G⁃四链体结构，该结构能够阻滞聚合酶链反应。结论原癌基因MET启动子形成的分子内G⁃四链体结构在细胞内K+条件下可能负性调控基因表达。%Objective To identify G⁃quadruplex structures in the promoter region of MET. Methods CD spectroscopy,UV spectroscopy,non⁃denatured electrophoresis and PCR stop assay were applied to indicate the G⁃quadruplex structure and its function. Results The Pu23WT se⁃quence in the promoter of MET adopted an intramolecular parallel G⁃quadruplex structure under physiological conditions in vitro,which can stop the extension of Pmet. Conclusion G⁃quadruplex structure in the promoter might inhibit MET gene expression in vivo.