目的 探讨抑制Rabll表达对膀胱癌T24细胞增殖和侵袭的影响.方法将Rabll siRNA转染于人膀胱癌T24细胞系,用免疫印迹法检测干扰效率.通过CCK8、细胞周期检测及基质胶侵袭实验,分析抑制Rabll表达对细胞增殖、细胞周期和侵袭的影响.通过免疫印迹法及实时PCR检测抑制Rabll表达对细胞周期相关因子cyclinDl、cyclinE及侵袭相关因子基质金属蛋白酶9(MMP9)表达量的影响.结果抑制Rabll蛋白表达能够抑制膀胱癌细胞的增殖和侵袭,并下调细胞周期相关因子cyclin Dl、 cyclinE及侵袭相关因子MMP9的蛋白和mRNA表达量.结论Rabll可能作为肿瘤蛋白参与膀胱癌的疾病进展.%Objective To investigate the effect of inhibition of Rabll expression on the proliferation and invasion of human bladder cancer cell line T24. Methods T24 cells were transfected with Rabll siRNA, and RNA interference efficiency was determined by performing Western blotting. The effect of inhibition of Rabll expression on cell proliferation, cell cycle progression, and cell invasion was analyzed by performing CCK8, cell cycle detection, and Matrigel invasion assays, respectively. Expression of cell cycle-related proteins cyclin D1 and cyclin E and invasion-related protein matrix metalloproteinase 9 (MMP9) was determined by performing Western blotting and RT-PCR. Results Inhibition of Rabl 1 expression inhibited the proliferation and invasion of bladder cancer cells and downregulated the expression of cell cycle-related proteins cyclin D1 and cyclin E and invasion-related protein MMP9. Conclusion Our results suggest that Rabl 1 functions as a tumor protein and is involved in the progression of bladder cancer.
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