基线等比增减设计法优选黄芪注射液及灯盏细辛注射液治疗气虚血瘀证小鼠联合用药配比∗

摘要

目的：考察不同配比的黄芪注射液、灯盏细辛注射液联合使用对气虚血瘀证小鼠凝血功能及血脂水平等的影响，优选出最佳配比。方法建立气虚血瘀模型小鼠，结合基线等比增减设计法，将实验小鼠分成正常对照组、模型对照组及黄芪-灯盏细辛注射液(10∶0、10∶3、10∶6、10∶10、1∶10、0∶10)配比组，观察给药前后小鼠体征变化，并检测小鼠凝血时间及血清中 TG、TC、LDL-C、HDL-C 水平。结果与模型组比较，除黄芪∶灯盏(10∶0)差异无统计学意义(P >0.05)外，其余各配比组均能显著延长凝血时间，差异有统计学意义(P <0.01或0.05)；各配比组除对 HDL-C 无明显影响外，均能显著降低 TG、TC、LDL-C 水平，差异有统计学意义(P <0.01或0.05)，均以黄芪∶灯盏(10∶6)组作用明显。结论黄芪-灯盏细辛注射液联用能改善小鼠的气虚血瘀症状，较单用效果好，最佳配比为黄芪∶灯盏(10∶6)。%Objective To observe the effect of different ratios of astragalus injection (AT)and Erigeron breviscapus injection (EB)on the mice with syndrome of Qi deficiency and blood stasis,and explore the optimum ratio.Methods The mouse models with syndrome of Qi deficiency and blood stasis were established and randomly divided into the control group,the model group,and the treatment groups with different AT-EB ratios including 10∶0,10∶3,10∶6,10∶10,1∶10,0∶10 on the basis of the increase-decrease baseline geometric proportion design method.Then the clotting time and the TG,TC,LDL-C and HDL-C levels of serum were detected.Results Compared with the model group,The treatment groups with different AT-EB ratios can significantly prolong the clotting time (P < 0.01 or 0.05 ) except that with the ratio of 10∶0.the level of HDL-C wasn′t changed significantly,but the levels of TG,TC and LDL-C decreased significantly in all the treatment groups with different ratios (P < 0.01 or 0.05 ),and the treatment group with the ratio 10∶6 showed better effect than the other treatments.Conclusion The combined therapy of astragalus injection and erigeron breviscapus injection can improve the syndrome of Qi deficiency and blood stasis in mice to different degrees,and the combined administration has better effect than the single one.The optimum ratio is 10∶6.