TNFRSF4基因 rs17568多态性与2型糖尿病的相关性

摘要

目的：探讨四川南充地区汉族人 TNFRSF4基因 rs17568多态性与2型糖尿病（T2DM ）的相关性。方法应用聚合酶链反应－限制性片段长度多态性技术检测186例健康体检者和220例 T2DM 患者 rs17568多态性。酶法测定血糖（Glu ）、血清甘油三酯（TG ）、总胆固醇（TC ）及高密度脂蛋白胆固醇（HDL‐C ）。结果TNFRSF4基因 rs17568位点等位基因频率和基因型频率分布均符合 Hardy‐Weinberg 平衡定律。rs17568多态位点G等位基因频率为28.2％。两组rs17568位点G等位基因的频率差异无统计学意义（ P＞0.05）。rs17568多态位点在T2DM组和对照组中，GG、AG基因型携带者血清 HDL‐C水平较 AA基因型携带者明显升高（P＜0.05）。结论 TNFRSF4基因 rs17568多态性与T2DM无关联，但其G等位基因与血清HDL‐C水平密切相关。%Objective To investigate the rs17568 polymorphisms of TNFRSF4 gene and their correlation with type 2 diabetes mellitus among people of Han race in the Nanchong area of Sichuan Province .Methods The genotypes and allele frequencies of rs17568 polymorphisms were assayed by polymerase chain reaction and restriction fragment length polymorphism (PCR‐RFLP) among 186 healthy subjects and 220 type 2 diabetes mellitus (T2DM ) patients .Then the enzymatic kits were used to assay their serum glucose (Glu) ,serum triglycerides (TG) ,total cholesterol (TC) and high density lipoprotein cholesterol (HDL‐C) .Results The allele and genotype frequencies at rs17568 site were in accordance with Hardy‐Weinberg equilibrium principle .The frequencies of G allele at rs17568 site were 0 .286 and 0 .276 in the T2DM group and the control group respectively and there was no significant difference in allele and genotypes frequencies between the two groups (P>0.05) .Subjects with genotype GG and AG at rs17568 sites had a higher serum concentration of HDL‐C than those with genotype AA in both groups (P<0.05) .Conclusion The rs17568 polymorphisms in the TNFRSF4 gene are not correlated with type 2 diabetes mellitus ,but the G allele at rs17568 site is correlated with serum high density lipoprotein cholesterol among people of Han race .