丝胶对2型糖尿病大鼠糖脂代谢及胰岛形态结构的影响

摘要

Objective:To investigate the effects of sericin on glucose and lipid metabolism and morphological structures of pancreatic islet. Methods:48 male SD rats were randomly divided into normal control group, diabetes mellitus model group, sericin treatment group and metformin group, with 12 rats in each group. The diabetes mellitus rat’s model was established by continuously injecting streptozotocin into the peritoneal cavity. After the rat’s model was successfully established, the rats in sericin treatment group and metformin group were respectively lavaged with sericin (2.4g/kg/d) and metformin (55.33mg/kg/d) for 35 days. The blood glucose and blood lipid of rats in each group were detected;HE staining was used to observe the morphological structures of pancreatic islet. Results:Compared with rats in model group, the blood glucose, triglyceride, total cholesterol, low density lipoprotein and free fatty acid of rats in sericin and metformin group decreased obviously, high density lipoprotein increased obviously (P<0.01). The pancreatic islet of rats in model group distributed sparse and uneven, the border was irregular, the cells of pancreatic islet decreased obviously. Compared with rats in model group, the morphological structure of pancreatic islet of rats in sericin group and metformin group improved obviously. Conclusions:Sericin can signiifcantly improve glucose and lipid metabolism and morphological structures of pancreatic islet of diabetes mellitus rats.%目的：探讨丝胶对2型糖尿病大鼠糖脂代谢和胰岛形态结构的影响。方法：雄性SD大鼠48只随机分为正常对照组、糖尿病模型组、丝胶治疗组和二甲双胍组，每组12只。腹腔注射链脲佐菌素建立糖尿病模型；待成模后，二甲双胍组、丝胶治疗组大鼠分别给予二甲双胍(55.33mg/kg/d)和丝胶(2.4g/kg/d)灌胃35d。检测各组大鼠的血糖和血脂，HE染色观察胰岛的形态结构。结果：与模型组大鼠相比，丝胶治疗组、二甲双胍组大鼠的血糖、甘油三酯、总胆固醇、低密度脂蛋白、游离脂肪酸明显降低，高密度脂蛋白明显升高，差异具有统计学意义(P＜0.01)。模型组大鼠胰岛分布稀疏且不均匀，轮廓欠圆润，边缘不规则，胰岛细胞数量减少；丝胶治疗组和二甲双胍组大鼠胰岛的形态结构明显改善。结论：丝胶可明显改善糖尿病大鼠的糖脂代谢和胰岛的形态结构。