血清β-羟丁酸测定方法的建立及其在rn糖尿病酮症酸中毒中的意义

摘要

目的：建立酶速率法测定血清β-羟丁酸，并探讨β-羟丁酸在糖尿病酮症酸中毒中的意义。方法：采用酶速率法在日立7170A全自动生化分析仪上测定60例正常人、30例糖尿病人和8例酮症患者血清中β-羟丁酸含量。结果：本方法测定β-羟丁酸回收率108%，线性范围0～4.0mmol.L-1，批内CV2.2%，批间CV2.6%;糖尿病酮症酸中毒患者血清β-羟丁酸明显升高。结论:采用酶速率法测定血清β-羟丁酸快速、准确、线性范围宽，可作为糖尿病酮症酸中毒特异和快速的诊断试验。%Objective　This study was to establish enzyme-rate method for determining serum beta-hydroxybutyric acid ,and to discuss its meaning in diabetes ketosis acidism. Methods　Enzyme-rate method was used to determine serum beta-hydroxybutyric acid in 60 cases of normals, 30 cases of diabetes ,and 8 cases of ketosis acidism by Hitachi 7170A autochemistry analyzer. Results　The recovery rate is 108%,the linerity extent 0-4.0 mmol.L-1, the coefficient of variation within-run is 2.2%and day-to-day is 2.6%. Conclusion　The enzyme-rate method for determining serum beta-hydroxybutyric acid save time, gives accurate results and has wide linerity,so it can used for diagnosis of diabetes ketosis acidism.