曲古抑菌素A单用及联用伊马替尼对K562R细胞增殖和凋亡的影响

摘要

目的:探讨曲古抑菌素A( trichostatin A,TSA)对耐伊马替尼细胞株K562R的增殖和凋亡的影响.方法:采用3-(4,5-二甲基-2-基)-5（3-羧基甲基苯基）-2-（4-磺酸苯基）-2H-四氮唑细胞毒试验法观察细胞增殖抑制,细胞凋亡率采用流式细胞仪凋亡试剂盒检测.结果:TSA单用能抑制K562R细胞的增殖,呈时间剂量依赖性,并能促进凋亡.与伊马替尼联用有明显协同作用.结论:TSA与伊马替尼联用具有明显抑制耐伊马替尼的K562R增殖和诱导凋亡的作用,可能为临床伊马替尼耐药的患者提供新的治疗药物.%Objective To explore the effect of trichostatin A (TSA) alone and combination with imatinib on the proliferation and apoptosis of K562R cells. Methods 3-(4,5-dimethylthiazol-2-yl) -5(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium( MTS) assay was used to observe the proliferation of K562R cells and apoptosis was analyzed by annexin-V/propidium iodide (PI) staining. Results After exposure to TSA, the proliferation of K562R cells was inhibited, and the effect was in both time- and dose-dependent manner. The apoptosis was induced. Combined with imatinib, the effect of proliferation inhibition was better. Conclusion TSA combined with imatinib can inhibit the proliferation of K562R cells and induce its apopotosis. TSA may become a new drug for imatinib-resistant patients.