eIF3 P170对发育心肌细胞周期的调控及其机制

摘要

Objective:To investigate the expression of eIF3P170, cdc2, cyclinB1 and cyclinD1 in developing cardiac myocytes, and the correlation between eIF3P170 with cdc2, cyclin D1, and cyclin B1 in mice.Methods:Mouse cardiac myocytes were obtained at different time points. RT-PCR was employed to detect the expression of eIF3P170, cdc2, cyclin D1 and cyclin B1 mRNA. n Results:Expressions of eIF3P170, cdc2, cyclinD1 and cyclinB1 mRNA were higher in the embryonic Day 13, 15, 18 and postnatal Day 1, 2, 3, 5. Expressions at postnatal Day 5 reached the highest (all P values<0.05 vs other time points), and then the expressions of these genes gradually decreased to the weakest at postnatal Day 30 (all P values<0.05 vs other time points). hTe mRNA expression of eIF3P170 was positively correlated with cdc2, cyclin D1 and cyclin B1 mRNA expression respectively. n Conclusion:hTe mRNA expressions of eIF3 P170, cdc2, cyclin D1 and cyclin B1 in the embryo and the early life atfer birth are high. hTey reach the maximum at postnatal Day 5, then gradually decreased.%目的：探讨eIF3 P170，cdc2，cyclinD1，cyclinB1在小鼠发育心肌细胞细胞周期中的表达及eIF3 P170与cdc2， cyclinD1，cyclinB1之间的相关关系。方法：取不同时间点小鼠心肌细胞，RT-PCR检测eIF3 P170，cdc2，cyclinD1和cyclinB1 mRNA的表达。结果：eIF3 P170，cdc2，cyclinD1和cyclinB1 mRNA的表达在胚胎第13，15，18天(G13，G15， G18)及小鼠出生后第1，2，3，5天(P1，P2，P3，P5)的表达均较强，在小鼠出生后第5天表达达到最强(P5与其他各时间点比较，P<0.05)，而后表达逐渐减弱，在出生后第30天(P30)的表达降至最弱(P30与其他各时间点比较， P<0.05)。eIF3 P170与cdc2，cyclinD1和cyclinB1 mRNA表达之间具有正相关关系。结论：eIF3 P170，cdc2，cyclinD1和cyclinB1 mRNA的表达在胚胎期及出生早期均较强，在小鼠出生后第5天达到最强，之后逐渐下降。