Aβ1-42海马注射对大鼠海马细胞的影响及黄精多糖的干预研究

摘要

Objective: To determine the effect of polygona-polysaccharose (PP) on learning and memory ability in rats with Alzheimer’s disease (AD). Methods: Forty ifve Sprague-Dawley rats were assigned into 3 groups. Rats in the sham-operated group were injected with normal saline. Rats in the Aβ group were injected with Aβ1-42. Rats in the PP group were injected with 16% PP solution for 45 days consecutively. hTe Morris water maze was used to investigate the ability of learning and memory in the rats. hTe effect of Aβ and PP on the hippocampus cells was observed by HE and Congo red staining of methanol. Results: Rats in the sham-operated group had no obvious morphological change; and morphology of rats in the PP group was basicaly normal. The layer of pyramidal cells in the Aβ group was decreased. hTe cells appeared sparse and irregular and became smaller. Karyopyknosis and vacuolardegeneration cells were also found. More positive staining materials aggradated in the Aβ group compared with the PP group by Congo red staining (P<0.05). Conclusion: Aβ infusion into the hippocampus results in the impairment of the neuronal degeneration in the rats, which shows similar characterizations of AD. PP can reduce the deposition of Aβ in the hippocampus.%目的：探讨Aβ1-42海马直接注射对大鼠海马细胞的影响及黄精多糖干预的作用。方法：将45只SD雄性大鼠分为3组：假手术组、Aβ模型组(Aβ组)和黄精多糖干预组(PP组)。Aβ组直接注射Aβ1-42于大鼠海马组织；PP组用16%黄精多糖溶液给予大鼠连续灌胃45 d。采用HE和甲醇刚果红染色观察Aβ及黄精多糖干预对海马组织细胞的影响。结果：海马的形态学在大鼠的假手术组没有显著变化，在PP组大鼠基本正常，Aβ组的锥体细胞层明显减少，细胞排列稀疏、不规则地变小，可见核固缩、空泡变性细胞。Aβ组相比于PP组，海马组织细胞出现明显Aβ沉积；Aβ组大鼠的阳性斑块数量高于PP组大鼠。结论：Aβ海马注射可以模拟大鼠海马组织阿尔茨海默病病理改变；黄精多糖可显著改善阿尔茨海默病大鼠海马的病理改变。