茶条槭SDH基因克隆及生物信息学分析

摘要

结合茶条槭转录组数据库，利用RT-PCR的方法克隆得到茶条槭SDH基因，命名为AgSDH，并对该基因及其蛋白进行了生物信息学分析。结果显示，AgSDH基因的ORF全长为1563 bp，共编码520个氨基酸，推测蛋白相对分子量为57.219 kDa，理论等电点为5.61，无信号肽，预测亚细胞定位于细胞质中。生物信息学分析表明AgSDH蛋白为亲水性蛋白，没有跨膜结构。蛋白质二级结构α-螺旋占35.38%，延伸链占18.65%，无规则卷曲占45.96%；三级结构预测表明AgSDH蛋白以单体形式存在。与目前已知的多种植物的SDH蛋白具有相似的结构功能域。系统进化分析表明，AgSDH蛋白与脐橙SDH蛋白亲缘关系最为接近。该研究结果为深入了解该基因的功能和茶条槭次生代谢产物合成的调控机理提供了基础数据。%AAcer ginnala Maxim SDH gene was cloned by RT-PCR from the transcriptome database of Acer palmatum. The gene namedAgSDH and the bioinformatics analysis of the gene and its protein was carried out. The results showed that the ORF ofAgSDH gene was 1 563 bp, encoding 520 amino acids. The relative molecular mass of theAgSDH gene was 57.219 kDa, the theoretical isoelectric point was 5.61, and no signal peptide was detected. The subcellular localization was predicted in cytoplasm. Bioinformatic analysis showed that the AgSDH protein was a hydrophilic protein with no transmembrane structure. The secondary structure analysis showed that AgSDH comprised 35.38% of alpha-helix, 18.65 extended strains and 45.96% random coil. The tertiary structure prediction indicated that the AgSDH protein existed as monomer. And has similar structural domain to the SDH proteins of many known plant species. Phelogenetic analysis showed that the closest relative of AgSDH was that ofCitrus clementinaamong known SDHs. The results provide the basic data for understanding the function of this gene and regulating mechanism of secondary metabolites synthesis.