非小细胞肺癌患者外周血Th1、Th2、Th17淋巴细胞及其相关细胞因子表达及临床意义

摘要

目的:观察非小细胞肺癌(NSCLC)患者外周血中Th1、Th2、Th17淋巴细胞及其细胞因子IFN-γ、IL-4、IL-17的表达及临床意义.方法:收集山西省肿瘤医院胸外科,原发未治疗的NSCLC60例患者作为实验组和60例健康志愿者作为对照组,以IFN-γ、IL-4、IL-17为代表分别测定Th1、Th2、Th17淋巴细胞因子.首先采用流式细胞技术(FCM)分别检测两组外周血Th1、Th2、Th17淋巴细胞含量,采用酶联免疫吸附法(ELISA)分别检测两组血清中细胞因子IFN-γ、IL-4、IL-17水平含量.同时分析比较两组外周血Th1、Th2、Th17淋巴细胞及细胞因子IFN-γ、IL-4、IL-17水平含量与临床病理特征关系.结果:实验组外周血Th1、Th2、Th17淋巴细胞的水平分别为(44.57±20.32)％、(3.08±1.28)％和(1.74±1.18)％;对照组外周血中Th1、Th2、Th17淋巴细胞的水平分别为(49.04±12.30)％、(2.67±1.36)％和(0.89±0.56)％,两组比较差异有统计学意义(P＜0.05).Ⅲ、Ⅳ期肺癌患者Th1淋巴细胞的水平(41.1±23.6)％、(44.6±20.3)％低于Ⅰ-Ⅱ期肺癌患者(46.2±19.1)％(P＜0.05),Ⅲ、Ⅳ期肺癌患者的Th2、Th17淋巴细胞水平高于Ⅰ-Ⅱ期肺癌患者(P＜0.05).鳞癌患者外周血Th2、Th17淋巴细胞水平高于腺癌患者.结论:检测NSCLC患者外周血Th1、Th2、Th17及其细胞因子IFN-γ、IL-4、IL-17水平含量对判断病情、了解机体的免疫功能具有重要的临床意义.%Objective:To study the expression and its clinical significance of Th1 、Th2 、Th17 lymphocyte and IFN-γ、IL-4、IL-17 related cytokine in peripheral blood of patients with non-small cell lung cancer (NSCLC).Methods:60 patients with primary and untreated NSCLC were enrolled and designed as experimental group,at the same time,60 healthy volunteers as control group.IFN-γ、IL-4、IL-17 were used as markers for the differentiation of Th1 、Th2 、Th17 lymphocyte subsets,respectively.Flow cytometry (FCM) was used for detecting the level of Th1 、Th2、Th17 lymphocyte of 2 groups.Enzyme linked immunosorbent assay (ELISA) was used for detecting the level of IFN-γ、IL-4 、IL-17 cytokine of 2 groups.Compared the levels of Th1 、Th2、Th17 lymphocyte and IFN-γ、IL-4 、IL-17 cytokine in peripheral blood of patients and analyzed two grounp relationship with clinicopathological characteristics.Results:The levels of Th1 、Th2 and Th17 lymphocytes in the peripheral blood of the experimental group were (44.57 ± 20.32) ％、(3.08 ± 1.28) ％ and (1.74 ± 1.18) ％,respectively;while those from the control group were (49.0 ± 12.3) ％、(2.7 ± 1.4) ％ and (0.9 ± 0.6) ％.There were significant difference in Th1 、Th2、Th17 lymphocyte and IFN-γ、IL-4、IL-17 cytokine between patients with stage Ⅲ、Ⅳ and those stage Ⅰ-Ⅱ (P ＜0.05).Conclusion:The detection of Th1 、Th2、Th17 lymphocyte and the levels of cytokines IFN-γ、IL-4、IL-17 in peripheral blood of patients with NSCLC have important clinical significance in judging the disease condition and understanding the immune function of the body.