麝香保心丸对心力衰竭大鼠肾脏血管紧张素Ⅱ受体各亚型表达的影响

摘要

Objective To explore the effect of Shexiangbaoxin pills (SXBXP) on expression of renal angiotensin II receptor ( ATR) subtypes in rats with heart failure. Methods Eighty male Wistar rats were randomly divided into control group (group A, n = 10) and sham operation group (group B, n = 10) . The heart failure model was established successfully in 35 of the rest 60 male Wistar rats. The 35 heart failure model rats were randomly divided into four group: heart failure model group (group C, n = 9) , medicine control group (group D, n = 8 ) , small dose of SXBXP group ( group E, n = 9) and large dose of SXBXP group ( group F, n = 9). The mRNA levels of ATR subtypes of renal cortex were measured by reverse transcription polymerase chain reaction (RT-PCR) in each group. Results Compared with group A, expression of AT,R and AT,R showed no significant difference in group B(P>0.05) , compared with group B, expression of AT, R was increased significantly in group C (P < 0.05 ) , expression of AT, R was decreased significantly in group C ( P < 0.01). Compared with group C, expression of AT, R was decreased significantly in group D, E and F( P <0.05 ) , expression of AT1 R was increased significantly in group D, E and F(P<0.01). Compared with group E, expression of AT, R was decreased significantly in group F(F<0. 05). Conclusion SXBXP regulated the expression of renal ATR subtypes, including the downregulation of AT, R and the upregulation of AT, R in rats with heart failure, and it is also effective to protect renal function and improve heart failure, especially large dose of SXBXP.%目的 探讨麝香保心丸对心力衰竭大鼠肾脏血管紧张素Ⅱ受体( angiotensinⅡreceptor,ATR)各亚型表达水平的影响.方法 选择雄性Wistar大鼠80只,采用抽签法随机分为正常对照组(A组,10只)、假手术组(B组,10只),其余60只制作心肌梗死后心力衰竭模型,35只大鼠模型制作成功,并采用抽签法随机分为模型组(C组,9只)、阳性药物对照组(D组,8只)、麝香保心丸小剂量组(E组,9只)和麝香保心丸大剂量组(F组,9只).取大鼠肾脏皮质,采用反转录聚合酶链反应(RT-PCR)法分别测定各组大鼠ATR各亚型mRNA表达水平.结果 B组与A组AT1R、AT2R比较,差异无统计学意义(P＞0.05)；与B组比较,C组大鼠AT1R表达明显上调,AT2R表达明显下调,差异均有统计学意义(P＜0.05)；与C组比较,D、E、F组大鼠AT1R表达明显下调,AT2R表达明显上调,差异有统计学意义(P＜0.01)；与E组比较,F组大鼠AT1R表达下调更显著(P＜0.05).结论 采用麝香保心丸治疗,可调节大鼠肾脏ATR各亚型的表达,使心力衰竭时上调的AT1R表达下调,下调的AT2R表达上调,对保护肾脏功能、改善心力衰竭起到了有益作用.同时,与小剂量麝香保心丸相比,大剂量麝香保心丸的疗效更为显著.