基于厚朴酚对白色念珠菌黏附性及其生物膜形成的影响探讨其抗龋作用

摘要

Objective To observe the impact of magnololon Candida albicans adhesion and biofilm formation in order to assess its anti-caries effect.Methods Through 96-well microtiter plate copy C.albicans in vitro adhesion and biofilm model, XTT reduction assay was applied to evaluate the effect of the different concentrations of magnolol on C.albicans adhesion and biofilm formation.Results Magnolol has inhibitory effect on C.albicans adhesion, its action has the time and the concentration dependence.The magnolol of 62.5 mg/L and 125 mg/L of magnolol had significantly inhibited adhesion of C.albicans, compared with the negative control group, the difference was statistically significant(P<0.05).Test the concentration of the selected five magnolol had different degrees of inhibition on C.albicans biofilms, of which the inhibition rates of 125 mg/L magnolol against C.albicans biofilm was 66.32%.Conclusion Magnolol can inhibit adhesion of C.albicans and reduce its pathogenicity, and has obvious inhibitory effect to the formed biofilm.The results suggest magnolol has a good application prospect as an anti-caries drug.%目的：观察厚朴酚对白色念珠菌黏附性及生物膜形成的影响，以探讨其能否用于治疗白色念珠菌相关龋病。方法运用微孔板法复制白色念珠菌体外黏附及生物膜模型，采用 XTT〔2，3-bis（2-methoxy-4-nitro-5-sulfophenyl）-2H-tetrazolium-5-carboxanilide〕减低法评价不同浓度厚朴酚对白色念珠菌黏附、生物膜形成的影响。结果厚朴酚对白色念珠菌的黏附具有抑制作用，其作用具有时间及浓度依赖性，62．5 mg／L和125 mg／L厚朴酚可以显著的抑制白色念珠菌的黏附，与阴性对照组相比，差异均有统计学意义（P＜0．05）；实验所选5个浓度的厚朴酚对白色念珠菌的生物膜均有不同程度的抑制作用，其中125 mg／L厚朴酚对生物膜的抑制率达到66．32％。结论厚朴酚可通过抑制白色念珠菌的黏附而有效降低其致病性，并对已形成的生物膜具有明显的抑制作用，提示其作为抗龋药物有良好的应用前景。