糖尿病患者血浆减少α-突触核蛋白的磷酸化和寡聚化

摘要

Objective To investigate the effect of the plasma of patients with type 2 diabetes (T2D) patient on the formation of oligomeric and phosphorylated α-synuclein (α-Syn).Methods A total of 70 hospitalized patients with T2D and 70 age-and sex-matched healthy control subjects were recruited.Blood plasmas were collected and recombinant human α-Syn was incubated with the plasma.ELISA was used to measure the levels of oligomeric and phosphorylated α-syn formed in the plasma.Results The levels of oligomeric and phosphorylated α-syn in the plasma of T2D patients were significantly lower than those in the plasma of healthy controls (P ＜ 0.05),which was positively correlated with age.Conclusion Compared with control plasma,the plasma from T2D patients decreases α-syn oligomerization and phosphorylation in an age-dependent manner.%目的 研究2型糖尿病(type 2 diabetes,T2D)患者血浆对α-突触核蛋白(α-synuclein,α-Syn)磷酸化和寡聚化聚集的影响.方法 收集首都医科大学宣武医院内分泌科T2D住院患者70例,另收集年龄和性别与之匹配的健康志愿者70例作为对照组.采集抗凝血,并分离血浆.将基因重组人α-Syn在患者和对照志愿者血浆中振荡孵育,ELISA法检测各组人群血浆中寡聚化和磷酸化α-Syn形成量.结果 T2D患者血浆中寡聚化和磷酸化α-Syn形成量较对照组均有显著降低(P＜0.05),且其与年龄有相关性.结论 T2D患者血浆以年龄依赖的方式减少寡聚化和磷酸化α-Syn的形成.