人类白细胞抗原Ⅱ类基因多态性与中国人群尘螨过敏性鼻炎相关性研究

摘要

目的 探索人类白细胞抗原Ⅱ类基因(human leukocyte antigen Ⅱ,HLA-Ⅱ)多态性在中国人群中尘螨过敏性鼻炎发病中的作用.方法 选取单纯尘螨过敏性鼻炎(allergic rhinitis,AR)病人71例,健康对照92例,提取受试者外周血DNA,应用聚合酶链反应序列分型法(polymerase chain reaction sequence-based genotyping,PCR-SBT)进行HLA-Ⅱ基因(DRB1、DQB1)分型.采用R软件进行HLA-Ⅱ基因频率统计及单倍体型分析包进行统计学分析,基因频率在AR与对照组中的比较应用x2及Fisher精确检验分析,结果进行Bonferroni多重矫正.结果 共检测到16个HLA-DRB1等位基因,13个HLA-DQB1等位基因.其中DQB1*06:01:01(P校正=0.010,OR=2.347,95％ CI:1.392 ～4.225)、DRB1*08:03:02(P校正=0.012,OR=3.213,95％ CI:1.732～7.821),在尘螨过敏性病人中的基因频率较健康对照组增加,差异具有统计学意义.结果提示,HLA-DRB1*08:03:02和HLA-DQB1*06:01:01等位基因可能是中国人群尘螨过敏性鼻炎发病的风险因子.单倍体型DRB1 * 08:03-DQB1*06:01(19％ vs 4.8％,P校正=0.007,OR=4.232,95％ CI:1.822～9.237)在病例组中频率增加.结论 HLA-DRB1*08:03:02和HLA-DQB1*06:01:01等位基因可能是中国人群尘螨过敏性鼻炎发病的风险因子.%Objective To elucidate the human leukocyte antigen]Ⅱ (HLA-Ⅱ) gene alleles associated with allergic rhinitis (AR) in house dust mites sensitive (HDM-sensitive) Han Chinese subjects.Methods Seventy-one patients with AR and 92 healthy subjects were recruited to this case control study.Frequencies of HLA were estimated by using the R statistical analysis package.Significance was evaluated usingx2 test and Fisher exact test.For multiple allelic frequency comparisons,Bonferroni correction was applied.Genotyping of HLA-DRB1 and HAL-DQB1 was performed by the polymerase chain reaction sequence-based genotyping method.Results A total of 16 alleles of the HL4-DRB1 locus and 13 alleles of the HLA-DQB1 locus were genotyped in the entire study cohort;of which the frequencies of alleles DQB1 * 06:01:01 (P =0.010,OR =2.347,95％ CI:1.392-4.225) and DRB1 *08:03:02 (P =0.012,OR =3.213,95％ CI:1.732-7.821) were significantly increased in HDM-sensitive AR patients compared to healthy controls and considered to be a risk factor for AR in Chinese Han subjects.The frequency of haplotype DRB1 * 08:03-DQB1 * 06:01 in HDM-sensitive AR group was also significantly higher than that in the control group (19％ vs 4.8％,P =0.007,OR =4.232;95％ CI:1.822-9.237).Conclusion HLA-DRB1 *08:03:02 and HL4-DQB1*06:01:01 are associated with mitessensitive AR and may confer a risk for development of AR in Han Chinese subjects sensitized to HDM.