ALOX15在慢性鼻窦炎伴鼻息肉中表达及糖皮质激素对其作用研究

摘要

目的 研究花生四烯酸酯15-脂氧合酶(arachidonate 15-1ipoxygenase,ALOX15)在嗜酸粒细胞型慢性鼻窦炎伴鼻息肉(eosinophilic chronic rhinosinusitis with nasal polyps,ECRSwNP)及非嗜酸粒细胞型慢性鼻窦炎伴鼻息肉(nonesinophilc chronic rhinosinusitis with nasal polyps,nonECRSwNP)中表达,并研究口服糖皮质激素对其表达的影响.方法 所有标本均由首都医科大学附属北京同仁医院标本库中获得,采用real-time PCR及免疫组织化学染色研究ALOX15表达与蛋白定位.比较激素治疗前ECRSwNP、nonECRSwNP及健康对照组中ALOX15 mRNA表达水平、蛋白定位及表达,并比较同一病人激素治疗前后mRNA及蛋白表达分布差异.结果 ECRSwNP组中ALOX15 mRNA及蛋白表达量高于健康对照组及nonECRSwNP组,差异有统计学意义(均P =0.000).健康对照组、nonECRSwNP组间差异无统计学意义(P =0.594,P =0.550).ALOX15蛋白表达于上皮细胞、浆细胞、中性粒细胞及嗜酸性粒细胞等多种细胞.ECRSwNP组激素治疗前后ALOX15 mRNA和蛋白表达差异有统计学意义(P=0.004,P=0.024).结论 ALOX15在ECRSwNP中表达显著高于nonECRSwNP及健康对照组,口服糖皮质激素1周能抑制其表达.%Objective To detect the expression of arachidonate 15-1ipoxygenase (ALOX15) in eosinophilic chronic rhinosinusitis with nasal polyps (ECRSwNP),and nonesinophilc chronic rhinosinusitis with nasal polyps (nonECRSwNP).Moreover,the effect of corticosteroid on ALOX15 expression was evaluated.Methods Samples were socked in RNA later and embedded in paraffin were acquired from library of Beijing Tongren Hospital.Real-time PCR and immunohistochemistry (IHC) were applied for mRNA quantification and protein distribution of ALOX15 in ECRSwNP,nonECRSwNP and healthy control group respectively.ALOX15 mRNA level of ECRSwNP samples pre-and post-corticosteroid treatment were detected.Results LOX15 mRNA level and protein expression were found increased significantly in ECRSwNP compared with nonECRSwNP and healthy control (both P =0.000).There was no significant difference between nonECRSwNP and healthy control (P =0.594,P =0.550).ALOX15 protein localized in a majority of cells including epithelia,plasma cells,neutrophils and eosinophils.One-week oral corticosteroid could decrease ALOX15 mRNA and protein expression in ECRSwNP significantly (P =0.004,P =0.024).Conclusion ALOX15 is upregulated in ECRSwNP compared with nonECRSwNP and control groups.Corticosteroid treatment could inhibit ALOX15 expression in ECRSwNP.