pH值和剂量对腹腔注射L-精氨酸诱导小鼠急性胰腺炎模型的影响

摘要

Objective To investigate the effects of different methods of L-arginine injection on acute pancreatitis model mice.Methods Mice suffering from acute pancreatitis were induced by different pH values and different doses of L-arginine,then mortality was calculated,and plasma amylase activity was detected by starch-iodine colorimetry,and pancreas and lung tissue injury severity was observed by paraffin section of HE staining at the time of 2,4,12,24 h after last injection.Results Mortality in piH11 high-dose group was 100％ at 2 h,and mice in other groups all survived.Compared with the control group,the plasma amylase activity and pancreas pathological score were significantly increased in all model groups (P ＜ 0.001).Compared with the other model groups,pancreas and lung tissue injury was the most severe in pH7 high-dose group.Conclusion The pH value and dosage of L-arginine solution have significant impacts on the degree of pancreatic injury.Acute pancreatitis mice model induced by 2 i.p.injections of L-arginine (400 mg/kg body weight) in 0.9％ sodium chloride (pH7) at 1-hour intervals was the most severe compared with the other model groups,and with high survival rate.%目的 探讨L-精氨酸(L-arginine)不同给药方法诱导小鼠急性胰腺炎模型的效果.方法 L-精氨酸溶液以不同pH值、不同剂量诱导小鼠急性胰腺炎模型,造模后2、4、12、24 h统计小鼠病死率,采集小鼠血浆以淀粉-碘比色法检测淀粉酶活性,取胰腺及肺组织行石蜡切片HE染色观察组织损伤严重程度.结果 pH值为11高剂量组小鼠2h病死率100％,其余各组各时间点小鼠均存活.3个模型组与对照组相比血浆淀粉酶活性、胰腺组织病理评分均升高,差异有统计学意义(P＜0.05).模型组之间比较,pH值为7高剂量组胰腺及肺组织损伤最重.结论 L-精氨酸溶液的pH值及给药剂量对胰腺损伤程度有不同程度的影响,20％L-精氨酸溶液pH值为7按4g/kg的剂量间隔1h连续2次腹腔注射诱导的小鼠急性胰腺炎模型组织损伤重,且小鼠存活率高.