紫外线照射未成熟树突状细胞诱导小鼠同种抗原免疫耐受及其机制研究

摘要

Objective To induce alloantigen immune tolerance between C3H mice and Balb/c mice by infusing the ultraviolet B(UVB)radiation immature dendritic cells (imDC) and and study the immunological mechanisms of this process.Methods (①) The authors induced alloantigen tolerance in C3H mice (H-2k) by intravenous injecting ultraviolet B (UVB) irradiated Balb/c immature dendritic cells (UVB-Balb/c imDC) derived from the cultures of Balb/c bone marrow cells.(②)Detection of immune tolerance induced regulatory T cells,interleukin-10 (IL-10) and interferon-γ(IFN-γ) in mice in order to study the tolerance mechanism.Results (①)C3H mice immunized with UVB-Balb/c imDC can produce immune tolerance to Balb/c antigens and can not produce anti-Balb/c antibody.(②)C3H mice immunized with UVB-Balb/c imDC can not clear the Balb/c spleen cells in vivo.(③)The T cells of C3H mice immunized with UVB-Balb/c imDC can secrete more IL-10 and produce more FOX-P3 + regulatory T cells than the control group in vitro.Conclusion (①)Immunization of C3H mice with UVB-Balb/c imDC allows C3H mice to fully tolerate Balb/c antigen.(②)The possible cause of immune tolerance to Balb/c antigens in C3H mice is due to the increased secretion of IL-10 from T cells and the production of more FOX-P3 + regulatory T cells.%目的 探讨应用紫外线照射未成熟树突状细胞诱导C3H小鼠对Balb/c小鼠同种抗原免疫耐受及其免疫学机制.方法 ①用紫外线B(ultraviolet B,UVB)照射Balb/c (H-2d)小鼠骨髓来源的未成熟树突状细胞(immature dendritic cells,imDC),诱导其凋亡,随后将其输注到C3H(H-2k)小鼠体内,诱导C3H小鼠对Balb/c抗原免疫耐受.②检测诱免疫耐受小鼠体内调节T细胞以及细胞因子白细胞介素-10(interleukin-10,IL-10)和干扰素-γ(interferon-γ,IFN-γ)分泌以研究其耐受机制.结果 ①UVB-Balb/c imDC免疫的C3H小鼠对Balb/c抗原产生了免疫耐受,不能产生抗Balb/c抗体.②免疫耐受C3H小鼠不能排异注射到体内的Balb/c脾细胞.③UVB-Balb/c imDC免疫的C3H小鼠T细胞分泌IL-10增加,并且产生更多的FOX-P3+调节T细胞.结论 ①应用UVB-Balb/c imDC免疫C3H小鼠可以使C3H小鼠对Balb/c抗原产生完全彻底的免疫耐受.②免疫耐受C3H小鼠对Balb/c抗原产生免疫耐受的可能原因是由于其T细胞分泌IL-10增加,并且产生更多的FOX-P3+调节T细胞.