中国北方汉族人群VEGF基因启动子区多态性的研究

摘要

Objective To investigate the vascular endothelial growth factor ( VEGF ) gene variations in the promoter region in Chinese Han population.Methods The polymorphisms of 300 Chinese Han healthy individuals from Northern China were analyzed using the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) or direct sequencing.The commercial statistics package SPPS11.5 was used to compare the distribution of the allele and the genotype, and to analyze their correlations with the data on European reported.Results Significant differences were observed between European and Chinese Han healthy individuals with regards to the frequencies of the genotype and allele for -2578C/A and -1154G/A ( P ＜ 0.01 ) Conclusion The frequency of -2578A/A,-1154G/G genotypes in Chinese Han population is significantly lower than that in European.%目的 研究中国北方汉族人群血管内皮生长因子(VEGF)基因启动子区多态性.方法 用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)或直接测序的方法 对北方汉族300名健康者进行多态性分型,采用SPSS 11.5统计学软件包进行等位基因和基因型分布的比较(与文献报道的欧洲人群相比).结果 中国北方汉族人群中VEGF启动子多态性位点-2578C/A和-1154G/A的基因型频率与等位基因频率,和欧洲人群相比有显著性差异(P<0.01).结论 中国北方汉族人群VEGF基因启动子区的多态性与欧洲人群不同,有统计学差异,其中-2578A/A、-1154G/G均低于欧洲人.