目的 探讨促红细胞生成素(EPO)对β淀粉样蛋白诱导大鼠海马CA1区神经元凋亡的影响及可能机制.方法 采用大鼠海马内注射β淀粉样蛋白(Aβ)制作阿尔茨海默病(AD)模型.将SD大鼠随机分为假手术组、生理盐水组及EPO处理组.Aβ大鼠海马内注射造模,然后在生理盐水组大鼠行脑室立体定向注射生理盐水,EPO处理组则行脑室立体定向注射重组人促红细胞生成素(rHu-EPO).观察术后24小时海马CA1区神经元细胞色素C(Cyt C)的变化,以及术后7天海马CA1区细胞凋亡变化.结果 EPO处理组大鼠海马CA1区神经元Cyt C的表达明显高于生理盐水组(P<0.01),EPO处理组大鼠海马CA1区凋亡细胞较生理盐水组减少(P<0.01). 结论 EPO可以通过抑制Aβ1-40诱导海马CA1区锥体细胞线粒体Cyt C释放来抑制神经元凋亡.%Objective To explore the effect of erythropoietin( EPO ) on rat hippocampal CA1 neuronal apoptosis induced by Amyloid beta-protein and the possible mechanism. Methods With Amyloid beta-protein ( Aβ )injected into rat hippocampus Alzheimer disease( AD ) models were made. Male Spraque-Dawley rats were as the experimental objects, which were randomly separated into 3 groups including Sham control, Saline control and EPO treatment. After Aβ injected into rats hippocampus CAI subregion, saline and EPO were respectively injected into the lateral ventricle of rats, with help of stereotaxic coordinates, upon the designed conditions. Changes of neuronal cytochrome c (CytC )in hippocampus CA1 subregion were observed 24 hours after the operation, and then changes of apoptosis in hippocampus CA1 subregion were observed 7 days after the operation. Results Hippocampal CA1 subregion showed the more CytC positive neurons in EPO group than in Saline group( P ＜ 0. 01 )and the EPO group had less apoptosis cells than the Saline control( P ＜ 0. 01 ). Conclusion EPO could restrain neuronal mitochondrial CytC releasing into cytoplasm and restrain neuronal apoptosis in the hippocampal CA1 subregion.
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