依达拉奉预处理对大鼠脑损伤后细胞毒性脑水肿的影响

摘要

Objective To investigate the potential therapeutic effect and the related mechanism of edaravone (EV) on rats with traumatic brain edema.Methods Thirty-eight Sprague Dawley rats were randomly divided into two groups:isotonic sodium chioride control group (Control group) and EV pretreatment group (EV group).The wet -dry weighing method was performed to measure the brain water content of rats with EV.The expression of aquaporin 4 (AQP4) and Na-K-2Cl Cotransporter-1 (NKCC1) was detected by Western blot.Results Compared with control group,EV significantly inhibited neurological dysfunction of rats in a concentration-dependent manner and decreased brain water content.It was suggested that EV could down-regulate the expression of AQP4 and NKCC1 to decrease the brain edema (P ＜ 0.05).Conclusion Pretreatment of EV could effectively protect traumatic brain edema whiEV is due to the regulation of AQP4 and NKCC1 expression.%目的探讨依达拉奉预处理对大鼠创伤性脑损伤的治疗作用及其机制.方法SD大鼠38只,随机分为生理盐水对照组(Control组)和依达拉奉预处理组(EV组).采用测干、湿重法检测不同剂量EV预处理对大鼠脑组织含水量的影响和Western-blot检测脑组织中水通道蛋白4(AQP4)及钠-钾-氯共转运体1(NKCC1)等蛋白表达情况.结果与生理盐水对照组比较,EV能明显减轻神经功能障碍,干、湿重法检测结果显示,EV明显减轻大鼠脑组织含水量,Western-blot结果提示EV下调AQP4、NKCC1蛋白的表达,组间差异有统计学意义(P＜0.05).结论EV预处理能减轻创伤性脑水肿,具有显著的神经保护作用,可能是通过调控AQP4和NKCC1而减轻脑组织水肿.