牛磺酸对大鼠局灶性脑缺血—再灌注损伤的全脑保护作用

摘要

Objective This study was conducted to investigate whether taurine(Tau)could be used to protect against focal ischemia/reperfusion damage and excert the efficacy of total brain protection.Method A thromboembolic model of transient focal cerebral ischemia/reperfusion was induced in the rat. Ischemic damage to neuronal perikarya was evaluated by hematoxylin and eosin-stained. Quantification of axonal and oligodendrocyte damage was determined with amyloid precursor protein and microtubule-associated protein tau-1 immunohistochemistry. ResultsTau significantly reduced the volume of neuronal perikaryal damage(P<0.01), and protected gray matter. Tau significantly reduced Total APP Score(P<0.01)and the volume of Tau-1 immunopositive oligodendrocyte (P<0.01), and protected axonal and oligodendrocyte in ischemic brain tissue.ConclusionTau protects gray matter and white matter in ischemic brain tissue from damage induced by focal cerebral ischemia/reperfusion and excerts the efficacy of total brain protection.%目的 探讨牛磺酸(Tau)对局灶性脑缺血—再灌注损伤全脑保护作用.方法 应用血栓栓塞法制作大鼠短暂性局灶性脑缺血—再灌注动物模型.应用苏木精—伊红染色方法研究神经元核周体损伤.应用免疫组组化学方法检测大鼠类淀粉样前体蛋白(APP)和微管相关蛋白tau—1表达,定量研究轴突和少突胶质细胞损伤.结果 牛磺酸能减小局灶性脑缺血—再灌注后神经元核周体损伤体积(P<0.01),保护灰质.牛磺酸能减小局灶性脑缺血—再灌注后缺血侧脑组织总的APP分数(P<0.01)和含tau—1免疫反应阳性少突胶质细胞体积(P<0.01),对轴突和少突胶质细胞有保护作用.结论 牛磺酸可减轻局灶性脑缺血—再灌注时脑缺血区灰质和白质的损伤,对局灶性脑缺血—再灌注损伤具有全脑保护作用.