大鼠胰十二指肠移植模型建立方法的改进

摘要

目的:改进现有的胰十二指肠移植模型的建立方法,建立一种符合生理模式的大鼠胰十二指肠移植模型.方法:运用显微外科技术,对链脲佐菌素诱导的糖尿病大鼠进行胰十二指肠移植.供胰的静脉回流采用符合生理的门静脉系统回流途径,即供胰的门静脉与受体肠系膜上静脉行端侧吻合.供胰的腹主动脉与受体腹主动脉行端侧吻合.供体十二指肠节断与受体十二指肠行侧侧吻合.结果:共进行50次大鼠胰十二指肠移植手术,术后血糖正常且存活超过5天者共39例,手术成功率为78%.结论:该模型稳定、可靠,符合生理模式,可用于进一步实验研究.%Objecave: To improve the method of pancreaticoduodenal transplantation and to establish a more physiological rat model. Methods: SD rats served as donors and recipients. The vein was reconstructed by end-to-side anastomosis between the donor portal vein and the recipient superior mesenteric vein, and arterial reconstruction was carried out by end-to-side anastomosis of the donor to the recipient abdominal aorta. Enteric drainage was performed by side-to-side anastomosis between the duodenum of donors and that of recipients. Results: Fifty experiments were performed. The successful rate of transplantation which restored the recipients euglycemia were 78%. Conclusion: This model of pancreaticoduodenal transplantation in rats was stable and reliable, which was in accordance with the trend of clinical pancreas transplantation and could be applied for further scientific research.