Since the parasite infection becomes a focused issue in the research of endangered waterbirds'conservation, it is urgent to get a simple, rapid and accurate method for parasite examination. The parasite eggs were separated from faecal samples, and treated the eggs by liquid nitrogen / 85℃ water bath, ultrasonication, and cracking fluid digestion, respectively, and then detected the differ-ent effects using real-time fluorescence PCR. The method used was very well to detect Capillaria by the eggs from Hooded crane faecals using real-time fluorescence PCR. The results showed cracking fluid digestion had the best effect, this method made the eggs to meet the need of molecular detection by one step pretreatment, providing stable and sensitive detection results with simple and efficient processes. It offers a reliable molecular detection for Capillaria, and also provids a convenient way for detection of the parasitic infec-tion in endangered wild bird species.%寄生虫感染是濒危水鸟保护关注的热点,简便、快速、准确的寄生虫检测方法是濒危野生水鸟寄生虫学研究亟待解决的问题. 为探索粪便寄生虫卵快速准确的鉴别方法,在越冬白头鹤粪便样品中寄生虫卵分离的基础上,通过对虫卵分别进行液氮/85℃水浴、超声破碎、裂解液消化等3种前处理,进行荧光PCR分子检测,建立粪便毛细线虫卵分子检测方法. 结果显示裂解液消化法处理效果最好,此方法使虫卵经一步预处理即可满足分子检测的需要,处理方法简便而有效,检测结果稳定而敏感. 此检测方法可为今后毛细线虫的分子检测提供可靠手段,也可以为濒危野生鸟类的寄生虫感染调查提供便捷的方法.
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