Sod1p缺失对酿酒酵母氟康唑、两性霉素B耐药性的影响

摘要

目的：研究酿酒酵母铜/锌超氧化物歧化酶基因SOD1对抗真菌药物氟康唑（ FLC）、两性霉素B（ AmB）耐药性的影响。方法采用平板滴定生长试验，检测酿酒酵母铜/锌超氧化物歧化酶缺失菌株（ sod1Δ）对FLC和AmB的药物敏感性；以及外源添加抗氧化剂N-乙酰半胱氨酸（ NAC）和谷胱甘肽（ GSH）对sod1Δ药物敏感性的影响。结果与野生菌株相比，sod1Δ菌株对FLC和AmB均表现出敏感性表型；外源添加NAC和GSH可恢复sod1Δ对FLC和AmB的耐药性。结论 SOD1基因和抗氧化能力在调控酿酒酵母FLC和AmB耐药性中发挥重要作用。%Objective To investigate the possible role of Cu ,Zn-superoxide dismutase gene SOD 1 on the resistance to antifungal drugs FLC and AmB in Saccharomyces cerevisiae .Methods The sensitivity of Cu , Zn-superoxide dismutase gene deletion mutant ( sod1Δ) to FLC and AmB were tested by spot plate growth assay .The effect of the antioxidants N-acetylcyseine ( NAC) and glutathione (GSH) on the sensitivity of to the sod1Δalso analyzed.Results Compared with the wild type yeast strain ,sod1Δmutant showed in-creased sensitivity to both FLC and AmB ,and this antifungal sensitivity of sod1Δcan be restored by the additions of antioxidants NAC and GSH, respectively.Conclusion The SOD1 gene and antioxiditive function are essential for the antifungal resistance of yeast to FLC and AmB.