正交试验法优选广痛消中芍药苷的提取工艺

摘要

Objective To optimize the extraction technique of paeoniflorin in Guangtongxiao Foam Aerosol. Methods Taking the content of paeoniflorin as index, the multiple of extraction solvent ( A), number of extraction (B) and period of extraction (C) were observed by using L9 ( 34 ) orthogonal design test for determining the best extraction technique. The HPLC procedure was performed on the chromatographic column of Agilent Extend (4. 6 mm × 150 mm, 5 μm) at 30℃ and the mobile phase was acetonitrile-0. 1％ phosphoric acid solution in gradient elution program (12:88 ). The flow velocity was 0. 8 mL/min and the detection wavelength was 230 nm. Results The sequence of influence of all factors on paeoniflorin extraction volume was A ＞ B ＞ C and the best extraction technique was A3B2C1 (adding water by 8 times, extracting for 2 times and for 1 hour each time). The extractive weight of paeoniflorin was 449. 502 2 mg, which was the most extraction quantity. Conclusion The technique is reasonable, stable and available, and provides a foundation for the further production and quality control of Guangtongxiao Foam Aerosol.%目的 优选广痛消泡沫气雾剂中芍药苷的提取工艺.方法 以芍药苷的含量为指标,采用L9(34)正交试验设计,对提取溶剂倍数(A)、提取次数(B)和提取时间(C)3个因素(每个因素设3个水平)进行考察,以确定最佳提取工艺.采用高效液相色谱法进行含量测定,Agilent extend色谱柱(4.6 mm×150 mm,5 μm),流动相为乙腈-0.1%磷酸水溶液(12:88),流速为0.8 mL/min,柱温30℃,检测波长为230 nm.结果 各因素对芍药苷提取量的影响大小顺序为A＞B＞C,最佳提取工艺为A3B2C1即加8倍水体积,提取2次,每次1 h.验证试验结果显示,芍药苷的提取重量为449.502 5 mg,提取量最多.结论 该工艺合理,且稳定可行,为以后的生产和质量控制奠定了基础.