温阳消饮法对 CHF 模型大鼠肾脏水通道蛋白2表达的影响

摘要

Objective To explore the potential mechanism of eliminating fluid-retention by yang-warming and fluid retention-resolving method.Methods Rat model of congestive heart failure (CHF)was estab-lished by intraperitoneal injection of adriamycin.After modeling,the CHF rats were randomly divided in-to model group,group of yang-warming and fluid retention-resolving formula (YWFRR group),and group of yang-warming and fluid retention-resolving formula minus cinnamon twig (YWFRR-CT group). The rats of YWFRR group were intragastrically administered (i.g.)YWFRR at the dose of 12 g/kg once daily for consecutive four weeks,and those of YWFRR-CT were i.g.YWFRR-CT at the dose of 10 g/kg once daily for four weeks,at the same time,the rats of model group and blank group took no medication treatment.Then the serum level of brain natriuretic peptide (BNP)and protein expression of aquaporin-2 (AQP2)in renal tissue were determined.Results Compared with the blank group ,the serum con-tent of BNP in model group increased significantly and the expression of AQP2 in renal tissue decreased significantly (P <0.05 or P <0.01).The contents of serum BNP were both lower in YWFRR group and YWFRR-CT group than that of model group,and the expression of AQP2 higher.Conclusion The method of yang-warming and fluid retention-resolving could decrease the serum content of BNP and upreg-ulate the expression of renal AQP2 of CHF rats.%目的：探讨温阳消饮法消除水饮的部分作用机制。方法Wistar 大鼠随机分为空白组和 A 组， A 组动物采用阿霉素（Adr）腹腔注射制备慢性心力衰竭（CHF）模型。造模结束后将 A 组大鼠再随机分为：模型组、温阳消饮组、去桂消饮组。①温阳消饮组：按12 g／（kg·d）等效剂量灌胃给予温阳消饮方药液，每日1次，连续4周；②去桂消饮组：按10 g／（kg·d）等效剂量灌胃给予去桂消饮方药液，每日1次，连续4周；③模型组、空白组：同等条件饲养，不实施任何给药方案。结果与空白组相比，模型组血清脑钠素（BNP）含量明显升高，肾脏组织中水通道蛋白2（AQP2）蛋白表达降低，差异有显著性（P ＜0．05或 P ＜0．01）；与模型组相比，温阳消饮组、去桂消饮组血清 BNP 含量明显降低，肾脏中AQP2蛋白表达增加，差异有显著性（P ＜0．05或 P ＜0．01）。结论温阳消饮法可显著降低 CHF 模型大鼠血清 BNP 含量，上调 CHF 模型大鼠肾组织 AQP2的表达。