采用序批式移动床生物膜反应器(MBBR),研究了以硝酸盐为电子受体的反硝化聚磷菌(DPB)的选择和富集.结果表明,采用3个阶段进行选择和富集,DPB占全部聚磷菌(PAOs)比例约从11.77％提高到66.07％；第3阶段培养末期,COD和TP去除率平均值分别为68.78％和69.02％,缺氧所耗ρ(NO3--N)达到23.91 mg/L；对反应器中生物膜进行直接染色发现,在厌氧放磷阶段能观察到聚磷菌体内有大量聚-β-羟丁酸(PHB)出现,而在缺氧吸磷阶段则有大量聚磷颗粒(Poly-p)出现,故可尝试采用聚磷生物膜的直接染色方法观察聚磷微生物细胞内PHB和Poly-p颗粒的变化,来判断生物除磷过程及效果.%The selection and dominance of denitrifying phosphorus removing bacteria (DPB) , which was capable of utilizing nitrate as electron acceptor, was investigated in a laboratory-scale sequencing batch moving-bed biofilm reactor (MBBR). The experimental results demonstrated that the proportion of DPB increased approximately from 11.77% to 66.07% of total phosphate accumulating organisms (PAOs) after three stages selection and dominance. In the end of the third cultivating period, average removal efficiencies of COD and TP were 68. 78% and 69. 02% , respectively, and a large quantity of nitrate, 23. 91 mg/L, was removed in anoxic phase. It was found that a great deal of PHB about PAOs emerged in anaerobic phase, whereas a mass of Poly-p about PAOs arose in anoxic phase by biofilm dyeing method, so they could be taken as evidences to judge the process and efficiency of biological phosphorus removal.
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