树莓遗传转化体系的建立与优化

摘要

[目的]树莓遗传转化体系的建立对研究其基因功能、性状形成机制等方面的生物学探索和育种改良方面的研究具有重要意义.[方法]建立2套稳定的遗传转化系统,系统1为叶片直接分化丛生芽遗传转化受体系统;系统2为树莓叶片脱分化形成愈伤组织再分化成芽遗传转化受体系统.[结果]转化效率分析结果显示,系统1转化率为25％,是系统2的2.1倍,抗性筛选获得阳性植株时间是系统2的50％.[结论]系统1具有更高的遗传转化效率.%[Objective] Establishment raspberry genetic transformation system has great significance for the study of its biological functions such as gene function,and breeding improvement.[Methods]In this study,two genetic transformation systems were established.One was the direct differentiation of clumpy bud genetically transformed receptor system from leaves.The other was dedifferentiation callus and then transformed into shoot genetic transformation receptor system from leaves.[Results] The results of transformation efficiency analysis showed that the conformation rate of system 1 was 25 ％ which was 2.1 times of system 2,and the positive plants obtained by resistance screening were 50 ％ compared with the system 2.[Conclusion]System 1 has a higher genetic transformation efficiency.