液质校准用利血平/甲醇水标准溶液的研制

摘要

研制了液相色谱-质谱( LC-MS)校准用利血平/甲醇水溶液标准物质。采用准确可靠的方法对利血平粉末纯度进行定值，用已确定值的利血平为溶质，甲醇水为溶剂，用重量-容量法制得利血平/甲醇水溶液。分别用F检验和回归曲线法对均匀性和稳定性进行考察，对定值结果的不确定度进行了评定。结果表明研制的利血平/甲醇水溶液标准物质具有良好的均匀性和稳定性，标准相对扩展不确定度为3%(k=2)。%Standard solution of reserpine in methanol aqueous for LC-MS calibration was developed. The puriety of reserpine powder was certified with credible methods. The standard solution was prepared by weight-volumetric method using the reserpine powder as solute and the methanol aqueous as solvent. Uniformity and stability of standard solution was analyzed by F test and regression curve method respec-tively, the uncertainty of the results was evaluated. The result showed that uniformity and stability of the standard solution of reserpine in methanol aqueous was better, the relative standard uncertainty of concen-tration was 3%( k=2 ) .