间断低压缺氧预处理对MCAO大鼠脑保护作用及对TLR4表达影响

摘要

目的 探讨间断低压缺氧预处理对MCAO大鼠的神经保护作用及对脑组织TLR4、NF-κB表达的影响.方法 实验大鼠分为:正常对照(C)组30只、假手术(S)组26只、单纯低氧处理(H)组35只,低压氧仓内间断低压处理、单纯大脑中动脉缺血(M)组43只,行单侧大脑中动脉栓塞处理 、预处理后脑梗死(HM)组37只;Longa评分,TTC染色及尼氏染色观察神经损伤程度,免疫组织化学法观察TLR4的细胞表达情况,Western blot法测定TLR4表达量,ELISA法测定TLR4及NF-κB表达变化.结果 HM组大鼠神经功能评分及梗死体积低于M组(P<0.05),残存神经元增多,TLR4表达下调(P<0.05),NF-κB表达下调(P<0.05).结论 低压缺氧预处理可有效减轻神经损伤,降低TLR4及NF-κB的表达.%Objective To investigate the neuroprotective effects of intermittent hypobaric hypoxia preconditioning on rats MCAO model ,and the change of Toll like receptor 4 and nuclear factor kappa B expression on brain tissue .Methods The rats were divided into control group ( C) with 30 rats;sham group ( S) with 26 rats,hypoxia treatment group ( H) with 35 rats which were put into a hypobaric chamber for intermittent hypobaric hypoxia treatment ;middle cerebral artery occlu-sion group (M) with 43 rats,hypobaric hypoxia preconditioning (HM) group 37,hypobaric hypoxia preconditioning before middle cerebral artery occlusion model;Longa score , TTC staining and Nissl staining were used to observe the degree of nerve injury immunohistochemistry staining method were used to observe the TLR 4 expression on cells ,ELISA and Western blot method for the determination of expression of TLR 4 and NF-κB on brain tissue .Results The degree of neurologic im-pairment in group HM was lighter than that in group M (P<0.05),and the number of surviving neurons increased and the expression of TLR4 and NF-κB was decreased (P<0.05).Conclusion Hypobaric hypoxia preconditioning can effectively alleviate nerve injury and reduce the expression of TLR 4 and NF-κB.