鸵鸟卵黄对猪精子冷冻后质量的影响

摘要

为了提高猪冷冻精液品质和精子抵抗低温打击的能力,本研究以5%、10%、15%、20%和25%等不同浓度的鸵鸟卵黄作为冷冻保护剂,以20%的鸡蛋卵黄和20%的鸽蛋卵黄为对照,将冷冻-解冻后的精子活率、质膜完整率和顶体完整率作为评价指标,分析鸵鸟卵黄对猪精子的抗冷冻保护作用。结果表明：稀释液中添加20%鸽蛋卵黄时,精子活率、顶体完整率和质膜完整性分别为52.11%、55.62%和54.94%,显著高于其他组（P〈0.05）。虽然稀释液中添加15%鸵鸟卵黄时,冷冻-解冻后精子活率、顶体完整率和质膜完整率显著高于5%、10%、20%和25%鸵鸟卵黄组,但仍然显著低于稀释液中添加20%鸽蛋卵黄处理组。本研究表明,鸵鸟卵黄在冷冻过程中对猪精子具有一定的保护作用,但相对于鸽子蛋和鸡蛋卵黄效果并不理想。%In order to improve the frozen semen quality and sperm resistance to cold shock,the different concentrations of 5%,10%,15%,20% and 25% ostrich egg yolk were added in extender as cryoprotectants,and 20% egg yolk and 20% pigeon egg yolk were control.The cryoprotective effects of ostrich egg yolk on the boar spermatozoa were analyzed and spermatozoa motility,acrosome integrity and membrane integrity after freezing-thawing were evaluated.The results indicated that the sperm motility,acrosome integrity,membrane integrity were 52.11%,55.62% and 54.94% respectively while the extender was supplied with 20% pigeon egg yolk,which was higher than that of other groups（P0.05）.Although sperm motility,acrosome integrity and membrane integrity in the extender supplemented with 15% ostrich egg yolk were significantly higher than that of 5%,10%,20% and 25% ostrich egg yolk groups,they were significantly lower than that of 20% pigeon egg yolk group.According to our study,the ostrich egg yolk could protect the boar spermatozoa during the frozen-thawed,but is not as effective as pigeon egg yolk and egg yolk.