碳离子辐照对甜高粱种子萌发及幼苗酶活的影响

摘要

[Objective] The aim was to study the effect of ~(12)C~(+6) ions beam irradiation to two varieties of Sorghum bicolor L. on seed germination and some enzymes activity in seedlings with different doses, and provided a theoretical foundation for breeding Sorghum bicolor. [Method] After germination, germination potential, germination fraction and enzyme activity were detected respectively. [Result] The results showed that with the dose increased, the germination potential of Sorghum bicolor increaseed first and then decreases, while their germination fraction presented "shoulder like shape"; the activity of LDH, SOD, CAT and GSH-Px increased first and then decreases with doses, they presented slight differences among different enzymes. [Conclusion] Low dose radiation could accelerate germination of sweet sorghum seeds and enzyme activity could remain at a relatively high level. Enzyme activity decreased with high doses and the growth of Sorghum bicolor was inhibited.%[目的] 研究辐射能量为100 MeV/u时不同剂量的碳离子辐照对2个甜高粱品种的种子萌发及几种酶活性的影响,为甜高粱品种的选育提供理论依据.[方法] 甜高粱种子萌发后,统计发芽势和成苗率,并测定幼苗叶片中几种酶的活性.[结果] 随着剂量的增加,发芽势先升后降,而成苗率逐渐下降,呈"类肩形";GSH-Px、CAT、SOD及LDH酶活性的总体趋势是随着剂量的增加先升后降,不同酶略有不同.[结论] 低剂量辐射促进了种子萌发,使酶的活性维持在较高的水平;高剂量时酶活下降,会抑制甜高粱的生长.