瑞昌山药组培快繁研究

摘要

[目的]探讨瑞昌山药组培快繁的技术环节.[方法]以瑞昌山药茎段为外植体,通过组织培养试验研究了不同激素及其浓度对愈伤组织形成、丛生芽诱导和生根的影响.[结果]70%乙醇和0.1%HgCl_2结合对外植体的的消毒效果最佳.培养基MS+6-BA 2.0 mg/L+NAA 0.2 mg/L中的腋芽生长最好,培养基MS+6-BA 1.0 mg/L+NAA 0.2 mg/L、MS+6-BA 1.0 mg/L+NAA 0.5 mg/L、MS+6-BA 2.0 mg/L+NAA 0.2 mg/L和MS+6-BA 2.0 mg/L+NAA 0.5 mg/L中腋芽的诱导率分别为52%、43%、84%和48%.在继代培养中,采用原诱导培养基诱导丛生芽的效果较差,降低6-BA的浓度有利于丛生芽的诱导.培养基1/2 MS+IBA 1.5 mg/L+NAA 0.3 mg/L+活性炭 0.2 mg/L中幼苗的生根效果较好.[结论]该研究为组织培养技术在种苗繁育中的应用奠定了基础.%[Objective]The aim of the research was to discuss the technical links in the tissue culture and rapid propagation of Ruichang yam. [Method]With the stem segments of Ruichang yam as explants, the effects of different hormones and their concn. on callus formation, tufted-bud induction and rooting were researched through tissue culture experiments. [Result]The disinfection efficacy of combing 70% alcohol with 0.1% HgCl_2 on the explants was best. The axillary buds grew best in the medium MS+6-BA 2.0 mg/L+NAA 0.2 mg/L, the induction rates of axillary buds in the media MS+6-BA 1.0 mg/L+NAA 0.2 mg/L, MS+6-BA 1.0 mg/L+NAA 0.5 mg/L, MS+6-BA 2.0 mg/L+NAA 0.2 mg/L and MS+6-BA 2.0 mg/L+NAA 0.5 mg/L were 52%,43%, 84% and 48% resp. In subculture, the efficacy of inducing tufted-bud with the original induction medium was worse and reducing the concn. of 6-BA was helpful to tufted-bud induction. The rooting efficacy of seedlings was better in the medium 1/2 MS+IBA 1.5 mg/L+NAA 0.3 mg/L+active carbon 0.2 mg/L . [Conclusion]The research laid a basis for the application of tissue culture technology in seedling breeding.