蓝靛果优良单株组培快繁技术的研究

摘要

[Objective] This paper aimed to study the tissue culture and rapid propagation technology of superior individuals of Lonicera edulis Turcz. [Method] Several superior individuals of Lonicera edulis Turcz were used as materials for selecting the primary medium, subculture medium, rooting medium and acclimatization substrate during the tissue culture and rapid propagation. [Result] 6-BA was the optimal cytokinin for tissue culture of Lonicera edulis Turcz, compared with ZT; modified MS＋1.0 mg/L of 6-BA ＋ 0.2 mg/L of IBA was the optimal medium as primary and subculture medium, modified MS＋ 1.5 mg/L of IBA was the optimal medium for rooting of Lonicera edulis Turcz, the rooting rate had achieved 100% after cultured for 30 d. The optimal substrate for transplanting plantlets of Lonicera edulis Turcz was composed of humus and perlite （1∶ 1, V/V）, survival rate was as high as 95% after 30 d. [Conclusion] This study provided basis for the rapid propagation of superior seedlings of Lonicera edulis Turcz, as well as the establishment of industrialized breeding technical system and the implementation of scale production.%[目的]对蓝靛果优良单株组培快繁技术进行研究。[方法]以蓝靛果几个优良单株为材料,对其组培快繁中的初代、继代和生根培养基及移栽基质进行筛选。[结果]与ZT相比,6-BA是较适合蓝靛果组培的细胞分裂素;改良MS＋6-BA1.0mg/L＋IBA0.2mg/L是较适合蓝靛果的初代和继代培养基;改良MS＋IBA1.5mg/L是较适合蓝靛果生根的培养基,生根培养30d,生根率达100%;较适合蓝靛果组培苗移栽的基质类型是腐殖土与珍珠岩体积比为1∶1的基质,移栽30d后的成活率达95%。[结论]为快速繁育蓝靛果优质苗木,建立工厂化育苗技术体系,进而实现规模化生产提供依据。