[目的]比较研究豆科植物中的D-松醇含量.[方法]采用高效液相色谱-蒸发光散射检测器( HPLC-ELSD)测定黄皮黄豆、绿皮黄豆、大豌豆、黑豆、红豆、豆角、荷兰豆、豌豆、毛豆、刀豆的D-松醇含量.[结果]D-松醇含量测定的标准曲线回归方程为:Y=1.421 1X+6.276 4,r=0.826 5,其进样质量线性范围为0.377～1.844μg.青豌豆荚的D-松醇含量为0.084％,青豌豆子实的D-松醇含量为0.339％,青荷兰豆子的D-松醇含量为0.190％,青荷兰豆子实的D-松醇含量为0.342％,青毛豆荚的D-松醇含量为0.760％,青毛豆籽实的D-松醇含量为1.135％,青长豆角的D-松醇含量为0.203％,荷兰豆的D-松醇含量为0.342％,黄皮大豆的D-松醇含量为0.198％,绿皮大豆的D-松醇含量为0.229％,黑皮大豆的D-松醇含量为0.319％,赤豆、豌豆和刀豆中未检出D-松醇.[结论]D-松醇在豆科植物中广泛存在,不同生长期其含量差别较大.%[Objective] The purpose was to compared and study the contents of D-pinitol in Legumina. [Method] The contents of D-pinitol in chrysochrous soybean, chlorochrous soybean, rounceval, black bean, ormosia, kidney bean, sweet broad pea, pea, green soybean and sword bean were determined by high performance liquid chromatography-evaporative light scattering detector ( HPLC-ELSD). [Result]The regression equation of standard curve of content determination of D-pinitol was Y =1.421 1X + 6. 276 4, r = 0. 826 5 and the linear range of its sample size was 0.377 - 1.844 μg. The contents of D-pinitol in green peasecod, green pea, green pod of sweet broad bean, green sweet broad bean, pod of green soybean, green soybean, green kidney bean, sweet broad bean, chrysochrous soybean, chlorochrous soybean and black bean were 0.084% , 0.339% , 0.190% , 0.342% , 0.760% , 1.135% , 0.203% , 0.342% , 0.198% , 0.229% and 0.319% resp. There was no D-pinitol detected in pea and sword bean. [Conclusion]D-pinitol existed in Legumina extensively and its contents of different growth periods had more difference.
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