[目的]研究迭鞘石斛(Dendrobinm chryseum Rolfe)所含多糖对小鼠脾淋巴细胞增殖以及腹腔巨噬细胞的影响.[方法]分离提取小鼠脾脏淋巴细胞和腹腔巨噬细胞,通过添加和不添加迭鞘石斛所含多糖进行体外培养,以MTT法检测这些免疫细胞增殖的变化情况.[结果]石斛多糖在中、高浓度时可极显著提高小鼠脾淋巴细胞和腹腔巨噬细胞的增值(P＜0.01),低浓度时没有增强作用.[结论]迭鞘石斛所含石斛多糖在体外对小鼠脾淋巴细胞增殖以及腹腔巨噬细胞的增殖有增强作用,说明对小鼠具有免疫增强作用.%[Objective] To study the effects of polysaccharides extracted from Dendrobinm chryseum Rolfe on the proliferation of mice spleen lymphocyte and peritoneal macrophages. [ Method] The mice spleen lymphocytes and peritoneal macrophages were cultured in vitro and treated with or without polysaccharides. The changes in proliferations of these immunocytes were detected with MTT. [ Result ] Middle and high concentration of polysaccharides could significantly increase the proliferation of mice spleen lymphocyte and peritoneal macrophages, while low concentration one had no enhancement effect. [ Conclusion ] Polysaccharides could promote the proliferation of mice spleen lymphocyte and peritoneal macrophages in vitro,suggesting that it was able to enhance the immunity of mice.
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