[目的]筛选苹果砧木耐盐变异体,并对其生理生化和分子生物学特征进行分析.[方法]用60Co-γ,对苹果砧木78-48进行辐射处理,以处理明显的叶片为外植体诱导出再生植株,以1.0%和1.2% NaCl作为选择压,对再生植株进行多代交替盐筛选,直到筛选出耐1.2% NaCl的变异体L和K,以耐盐变异体12和K2为试材,对其进行耐盐生理生化指标测定及RAPD分析.[结果]变异体不仅在生理生化指标上有明显的差异,而且在DNA水平上发生了突变.[结论]变异体L2和K2是78-48的耐盐新株系.%[ Objective] The research aimed to screen the salt- tolerant mutant of apple rootstock, and analyze their physiological- biochemistrical and molecular biological characteristics. [ Method] The original line 78-48(CK) was irradiated by 60Co-γ, and the effect obvious leaves were chose to induce the regenerative plants, then the plants were alternating salt screened taking 1.0％ and 1.2％ NaC1 as selection press,until to screen the mutants to 1.2％ NaC1- tolerance. Finally, the physiological- biochemistrical indices of the salt- tolerant mutants, L2 and K2, were determined, and their molecular biological characteristics were studied by RAPD analysis. [ Result ] The result showed that compared with the original line, the mutants varied not only on the salt-tolerant indexes level, but also on the DNA level, which provided a strong evidence for the truth of the mutants. [ Conclusion] These mutants ( L2 and K2 ) were salt-tolerant new lines of apple rootstock.
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