罗格列酮和血清对猪前脂肪细胞诱导分化过程中PPARα和PPARγ基因表达的影响

摘要

[ Objective ] The research aimed to discuss the effects of rosigli tazone and serum on PPARα and PPARγ gene expressions in the induced differentiation process of pig preadipocyte. [ Method ] The pig preadipocyte was separated by using the collagenase digestion method.Three kinds of different differentiation culture fluids were used to induce the differentiation of pig preadipocyte. The oil red O staining extraction method was used to contrast the influences of different differentiation culture fluids on the cellular fat content variation in the differentiation process. Moreover, the variation trends of PPARα and PPARγ gene expressions in the cellular differentiation process in the different differentiation culture fluids were detected by RT-PCR. [ Result] The cellular fat accumulation was the fastest in Mll which had rosiglitazone and was the slowest in MI which didn' t have rosiglitazone. Rosiglitazone could extremely significantly increase the expression of PPARγ gene ( P ＜0.01 ), but had the certain inhibition effect on the expression of PPARα gene, which wasn't significant. The serum had the extremely significant up-regulation effect on the expression of PPARγ gene ( P ＜ 0.01 ), but had the extremely significant downward effect on the expression of PPARα gene (P ＜ 0. Ol ). [ Conclusion] Rosiglitazone could greatly promote the expression of PPARγ gene, which increased the cellular fat deposition. Maybe the activator of PPARγ gene existed in the serum, and the inhibitor of PPARα gene simultaneously existed.%[目的]探讨罗格列酮和血清时猪前脂肪细胞诱导分化过程中PPARα和PPAγ基因表达的影响.[方法]利用胶原酶消化法分离了猪皮下前脂肪细胞,采用3种不同分化培养液对猪前脂肪细胞进行了诱导分化,借助油红O染色提取法比较了不同分化培养液对分化过程中细胞内脂肪含量变化的影响,并运用实时定量RT-PCR方法检测了不同分化培养液细胞分化过程中PPARα和PPARγ基因表达的变化趋势.[结果]细胞内脂肪聚积以舍罗格列酮的MⅡ最快,不含罗格列酮的MI最慢.罗格列酮可极显著上调PPARγ基因的表达(P<0.01),而对PPARα基因的表达存在一定的抑制作用,但不显著.血清对PPARγ基因的表达有极显著的上调作用(P<0.01),而对PPARα基因的表达有极显著下调作用(P<0.01).[结论]罗格列酮可极大地促进PPARγ基因的表达,继而增进细胞内脂肪沉积;血清中可能存在PPARγ基因的激活剂,同时存在PPARα基因的抑制因子.