鸡前脂肪细胞中A-FABP基因表达的变化对PPARγ、perilipin和E-FABP表达的影响

摘要

This study was designed to investigate the role of chicken A-FABP gene in lipid metab-olism of adipocytes and the regulation relationship between AFABP gene and the genes related to lipid metabolism. The shRNA interference vector with A-FABP gene and eukaryotic expres-sion vector were used. Chicken adipocytes with short hairpin RNA-mediated knockdown and A-FABP cDNA overexpression were used. The genes expression level in chicken adipocytes were detected at 24, 36, 48, 60 and 72 h after transfection with the interference and overexpression plasmids of AFABP. The results showed that the expression levels of PPARy and perilipin genes were downregulated at 24 and 36 h and upregulated at 48 h in the A-FABP interference group. The expression levels of E-FABP genes was downregulated at 48 and 72 h in the A-FABP interference group. The expression levels of PPARy and perilipin genes were upregulated at 24 and 36 h and downregulated at 48 h in the A-FABP overexpression group. The expression levels of E-FABP genes was downregulated at all detected time pionts in the A-FABP overexpression group. These results indicated that A-FABP might affect expression of PPARy, perilipin and E-FABP in chicken adipocytes.%本研究以鸡原代前脂肪细胞为实验材料,探讨A-FABP基因在鸡脂类代谢过程中的功能以及该基因与脂类代谢相关7个基因间的调控关系.利用A-FABP基因的shRNA干扰载体和真核表达载体对该基因进行干扰和过表达,分别在干扰和过表达后24、36、48、60和72 h时检测脂类代谢相关基因的表达情况.结果表明,干扰A-FABP后,鸡前脂肪细胞中PPARγ和perilipin基因的表达量在24和36 h时显著低于对照组,在48 h时显著高于对照组,在48和72 h时,E-FABP基因表达量显著低于对照组；过表达A-FABP后,鸡前脂肪细胞中PPARγ和perilipin基因的表达量在24和36 h时显著高于对照组,在48 h时显著低于对照组,在所有检测的时间点E-FABP基因表达均显著下调.结果显示,在前脂肪细胞中,鸡A-FABP表达量的变化影响PPARγ、perilipin和E-FABP的表达.