透明质酸发酵过程中产物抑制研究

摘要

[目的]寻求得到高产量透明质酸的发酵方法.[方法]在微生物发酵法生产透明质酸的过程中,用碱液调节pH对其产物抑制进行研究.[结果]在发酵过程中定时用碱液调节pH,可解除产物抑制,提高透明质酸的产量,通过试验确定发酵过程中需调节6次pH,时间分别为8、12、16、20、24、28 h.[结论]在微生物发酵过程中,定时用碱液调节pH可以促进兽疫链球菌菌体的生长和透明质酸的产生.%[ Objective ] The aim of the study was to seek a good method of·fermentation to get high yields of hyaluronic acid. ［ Method ] Product inhibition by adjusting pH with alkali in the microbial fermentation was studied. [ Result] Adjusting pH with alkali at regular time during fermentation,could disarm product inhibition and improve hyaluronic acid production. The exploratory experiment gave the number of adjusting pH as many as 6 times :8th hour, 12th hour, 16th hour,20th hour,24th hour and 28th hour. [ Conclusion ] It could promote the growth of streptococcus zooepidemicus bacteria and hyaluronic acid production,by adjusting pH with alkali at regular time during the microbial fermentation.