香蕉品种(系)遗传多样性的SSR分析

摘要

[目的]从分子水平上研究香蕉种质资源的遗传多样性和亲缘关系,建立分子水平香蕉分类系统.[方法]应用SSR 技术从分子水平上对32 个香蕉品种(系)的遗传关系进行检测.[结果] 39对SSR引物在32个品种(系)中扩增带数在4～20个,平均每个SSR 座位可检测2.78个多态性带.引物的多态信息量(PIC)在0.45-0.91,平均0.80.依据SSR 数据计算的品种间遗传距离在3.06%-43.61%,平均30.73%.[结论] 香蕉品种之间存在较为丰富的遗传变异.%[ Objective ] The genetic diversity and relationship of the banana germplasms were analyzed at the molecular level in order to establish the classification system of bananas at molecular level. [ Method] The genetic relationship of 32 banana varieties(lines) was detected with the SSR technology. [ Results] The number of amplified band of 32 varieties (lines) based on 39 pairs of SSR primers was ranged from 4 to 20 ,the average 2.78 SSR polymorphic bands could be detected in each primer site and the polymorphism information content (PIC) was 0.45 -0.91 ( average value of 0.80). The genetic distance among varieties was 3.06％ - 43.61％ (30.73 ％ on average) according to the calculation of SSR data. [ Conclusion ] There was the relatively rich genetic variation among banana cultivars.