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暗紫红毛菜ITS序列的测定与分析

     

摘要

[ Objective ] To providing new data for phylogenesis of Bangia atropturpurea by the sequencing of internal transcribed spacer (ITS region) uf rDNA. [ Method ] B. atropurpurea was collected from Niangziguan Spring in Shanxi, the DNA was extracted and the primers were designed for PCR amplification so as to obtain ITS gene sequence. [ Result ] The bomology of ITS region between B.atropurpwrea and Porfihym oligaspermataangia was 75% , which was 79% between B.asropurpurea and P.yewensis.[ Conclusion ] Compared with other genes, the ITS sequence had a greater evolution rate. The differences in classification and distribution resulted in the sequence diversity.%[目的]对暗紫红毛菜rDNA内转录间隔区(ITS区)进行序列测定,为其系统发育研究提供新资料.[方法]以产于山西娘子关泉的一株暗紫红毛菜为材料,提取DNA,设计引物,进行PCR扩增,进而测定其ITS区基因序列.[结果]暗紫红毛菜与同科的少精紫菜ITS区同源性为75%,与条斑紫菜ITS区同源性为79%.[结论]ITS区序列进化速率相对较快,种类和地理分布的差异是其序列差异产生的原因.

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