自由基清除剂对香石竹切花瓶插寿命及生理效应的影响

摘要

[目的]探讨自由基清除剂对香石竹切花瓶插寿命及生理效应的影响.[方法]以高温季节的香石竹切花为试材,以8-HQ和蔗糖为基础保鲜物质,分别添加2种自由基清除剂(MA和SBN)和外源活性氧ST,设置4个处理,以蒸馏水为对照,探讨自由基清除剂用在香石竹切花瓶插过程中SOD、CAT及O<,2><'->等生理指标的变化规律.[结果]各处理的香石竹瓶插寿命均比对照长,其中MA处理最为明显,比对照延长了6.5 d;MA和SBN处理对O<,2><'->达到峰值的时间分别比对照推迟6.0和8.0 d,对SOD达到峰值的时间比对照延长2.0d,对CAT达到峰值的时间分别比对照延长8.0和6.0 d.[结论]2种自由基清除剂的处理在香石竹切花瓶插过程中对抑制O<,2><'->的水平及促进SOD和CAT的合成具有明显效果,并能明显减缓SOD和CAT活性下降的速率.%[ Objective ] The study aimed to discuss the effects of free radical scavenging agents on the vase life of Dianthus caryophyllus cut-flowers and its physiological action. [ Method] With D. caryophyllus cut-flower in the high temperature season as the tested materials, 8-HQ and sucrose were taken as the basic preservation material and 2 kinds of free radical scavenging agents ( MA and SBN ) and the exogenous active oxygen ST were added in them to set up the 4 treatments with the distilled water as the CK, thus to discuss the change rule of physiological indexes such as SOD, CAT, and O2 - in the vase process of D. caryophyllus cut-flowers by using free radical scavenging agents. [ Result]The vase life of D.caryophyllus in each treatment was longer than CK, in which the MA treatment extended the vase life by 6. 5 d than CK. As for MA and SBN treatment compared with CK, the time of O2 - reaching peak value was delayed by 6.0 and 8.0 d resp. , the time of SOD reaching peak value was delayed by 2.0 d and the time of CAT reaching peak value was delayed by 8.0 and 6.0 d resp. [ Conclusion ] In the vase process of D. caryophyllus cut-flowers, the treatments of 2 kinds of free radical scavenging agents had obvious effects on inhibiting the O2 - level and promoting the synthesis of SOD and CAT significantly and could significantly slow the decline rate of SOD and CAT activity.