麦冬生育年限对黄酮含量的影响

摘要

[目的]研究不同生育年限的杭麦冬商品块根黄酮含量的相关性规律,确定其品质特征,为麦冬GAP质量控制及资源综合开发利用提供科学依据.[方法]在杭麦冬(3年生)主产区采用原位动态法,按照麦冬栽种后0.5、1、2和3年分别随机采集植株块根样品,将洗净的鲜块根微波杀酶-50℃恒温烘干,采用紫外分光光度法分别测定各处理麦冬块根的黄酮含量,并运用SPSS17.0版统计软件分析不同生育年限的麦冬块根黄酮含量的相关性规律.[结果]不同生育年限杭麦冬块根的黄酮平均含量为17.68％～34.86％；随着麦冬植株块根生长发育进程增加,麦冬块根的黄酮含量呈极显著的正相关增长(P ＜0.001),其回归方程分别为:生育年限y1=-0.008x2+0.066x+0.046(R2 =0.999),生育时间y2=-5E-08 x2+0.047(R2 =0.998).[结论]麦冬块根生育年限与次生代谢产物黄酮的含量密切相关,杭麦冬主产区3年采收是有科学依据的,川麦冬道地主产区由于气候的特殊性,第2年夏秋高温潮湿易导致上年块根大量腐烂,只适宜于1年采收.%[Objective] To research the effects of growth period on the flavoniods content of RADIX OPHIOPOGONIS, and to provide scientific references for the GAP quality control of RADIX OPHIOPOGONIS and development and utilization RADIX OPHIOPOGONIS resources. [Method] Production area of three-year-old RADIX OPHIOPOGONIS in Hangzhou City was studied by in-situ dynamic method. Random collection of Ophiopogon japonicus root tuber was conducted after 0.5, 1,2 and 3 years of cultivation. After washing, the 0. Japonicus fresh root tuber was dried at -50 T. Flavoniods contents of 0. Japonicus dry root tubers (RADIX OPHIOPOGONIS) under different treatments were detected by UV spectrophotometry. Correlation rules of the flavoniods contents in RADIX OPHIOPOGONIS with different growth years were analyzed by SPSS17. 0 software. [Conclusion] The average content of flavoniods in RADIX OPHIOPOGONIS was between 17. 68% and 34. 86%. With the increase of RADIX OPHIOPOGONIS growth years, flavoniods content showed extremely significant positive increase (P < 0.001). The regression equation was y, = - 0. 008x2 + 0. 066x + 0. 046 (R2=0.999), and y2 = -5E-08x2 +0. 047 (K2=0.998). [Con-clusion] Growth years of RADIX OPHIOPOGONIS had close correlation with the content of flavoniods. There was scientific basis for the collection of three-year-old O. Japonicus in main production areas. Due to the particular climate in the main production areas, root tubers should be collected at one year old; and they were easily decayed in the summer at the second year due to the high temperature and humidity.