[ Objective] The research aimed to find out candidate major genes for regulating the wool traits from immune genes. [ Method] U-sing microarray technology, the differential expression of immune genes between body side skin and groin skin of Aohan fine wool sheep was detected. [Result] 46 immune genes (difference multiple) >2.0) were identified and classified. And 6 of them were validated by real-time quantitative PCR. The results showed that the consistency between microarray data and real-time quantitative PCR results was 66. 67% , which indicated that the microarray data was reliable. [Conclusion] The mechanism might be involved in the growth regulation of wool.%[目的]从免疫基因中挖掘调控羊毛性状的候选主效基因.[方法]利用芯片技术,检测敖汉细毛羊体侧部与腹股沟部皮肤免疫基因的差异表达.[结果]差异倍数在2.0以上的免疫基因共46个,并对其中6个基因进行了实时定量PCR验证,其结果表明芯片数据和实时定量PCR验证结果相符率达66.67％,说明芯片数据是可靠的.[结论]免疫赦免机制可能参与羊毛的生长调控.
展开▼
