[Objective] To compare and find out the best online detecting method for resveratrol.[Method] Two methods have been compared , high performance liquid chromatography (HPLC) and UV spectrophotometric, for the determination of resveratrol in peanut root by ex* periment. [ Result] The linear correlation R was 0. 999 6 for UV method and 0. 999 8 for HPLC method, both two methods had good linearity and were all applicable to detecting the resveratrol content in peanut root. The precision RSD was 1. 87% for HPLC and 2, 52% for UV, and the rate of recovery was 98.8% -101.2% for HPLC with RSD of 1. 25% and 97.8% -103,5% for UV with RSD of 2,25% t as seen above, HPLC method was better than the UV method, from an average of the measured results, the resveratrol was 0. 699 1% detected by UV method with RSD = 2. 52% (n = 4) , and it was 0. 690 4% detected by the HPLC method with RSD = 1. 87% (n - 4), the detection results of two methods were similar. [Conclusion] Taking into account the wide applicability of online monitoring and instruments, the ultraviolet spectre-, photometric method has more advantages in detecting the content of resveratrol in peanut root.%[目的]比较并筛选出适用于白藜芦醇在线检测的方法.[方法]通过试验对比了高效液相色谱(HPLC)和紫外分光光度(UV)法测定花生根白藜芦醇的异同.[结果]从线性相关性看,R(UV) =0.9996,R(HPLC) =0.9998,2种方法都有良好的线性度,都适用于花生根白藜芦醇的检测;从精密度[ RSD(HPLC)=1.87％,RSD(UV) =2.52％]和回收率[HPLC 98.8％ ～ 101.2％,RSD为1.25％;UV法97.8％ ～ 103.5％,RSD为2.25％]看,HPLC法优于紫外分光光度法；从平均测得结果看,紫外分光光度法测得白藜芦醇含量为0.6991％、RSD =2.52％ (n =4),HPLC法测得白藜芦醇含量为0.6904％,RSD=1.87％ (n =4),2种方法结果相近.[结论]本着在线监测和仪器的普适性,紫外分光光度法更有优势,更适用于花生根白藜芦醇的检测.
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