GmSARK启动子驱动IPT基因在拟南芥中的表达研究

摘要

[Objective] This study aimed to investigate the expression of IPT gene directed by GmSARK promoter in Arabidopsis. [ Method] IPT gene and GmSARK promoter were respectively cloned to construct plant expression vector and transform Arabidopsis. T, transgenic plant lines were detected by PPT(phosphinothricin) herbicide selection and treated under drought and dark conditions for semi-quantitive RT-PCR analysis. [ Result] GmSARK and IPT fusion gene was successfully cloned and the plant expression vector p3301 -GmSAHK-IPT was constructed. RT-PCR analysis showed that the target gene was expressed in T1 transgenic plant lines at the mRNA level. [ Conclusion] This study laid the foundation for further investigating the roles of IPT gene directed by GmSARK promoter in plant stress resistance.%[目的]对GmSARK启动子驱动IPT基因在拟南芥中的表达进行研究.[方法]分别克隆IPT基因及GmSARK基因启动子,构建它们的植物表达栽体并进行拟南芥的转化,利用PPT(phosphinothricin)除草剂筛选,检测T1代转基因植株；对T1代转基因植株进行黑暗避光和干旱处理后进行半定量RT-PCR分析.[结果]成功克隆得到了IPT基因及GmSARK基因,并构建了它们的p3301 -GmSARK-IPT植物表达载体；对T代转基因植株的RT-PCR表明,目的基因mRNA水平上有所表达.[结论]为进一步研究GmSARK启动子驱动的IPT基因在抗逆中的作用奠定了基础.