HPLC法测定红树莓籽中鞣花酸含量

摘要

[Objective] The purpose was to determined content of ellagic acid extracted from red raspberry seed by HPLC. [ Method] The chromatography conditions were as follows; C18 column of ODS HYPERSL (100. 0 mm ×4. 6 mm at 5 μm) , column temperature of 25 ℃, CH3OH - 0.03% trifluoroacetic acid ( volume ratio of 50: 50) as mobile phase and detection wavelength of 254 nm. [ Result ] The results showed that there was a good linearity between the peak area and the content of ellagic acid in the range of 0. 322 - 8.050 μg( r = 0.999 5). Precision test' s RSD was 0.69% , showing instrument had good precision; Reproducibility test' s RSD was 0. 95% , showing determination method had good reproducibility; Average recovery of sample was 93. 78% , RSD was 1. 08% , showing determination method was accurate and reliable, could be used in subsequent analysis. And finally, the content of ellagic acid extracted from red raspberry seed was up to 994 mg/kg. [ Conclusion] The content of ellagic acid in red raspbeny seed is higher than that reported in the red raspberry fruit and its mechanism should be further studied.%[目的]利用高效液相色谱法(HPLC)测定红树莓(Rubus corchorifolius Linn.f.)籽提取物中鞣花酸的含量.[方法]色谱条件:ODSHYPERSL C18柱(100.0 mm ×4.6mm,5 μm),柱温25℃,流动相CH3OH-0.03％三氟乙酸(体积比50∶50),检测波长254 nm,用外标法测定鞣花酸含量.[结果]鞣花酸含量在0.322～8.050μg范围内与峰面积呈良好的线性关系,r=0.9995；精密度试验RSD为0.69％,表明仪器精密度良好；重现性试验RSD为0.95％,表明方法的重现性良好；平均加样回收率为95.32％,RSD为1.08％,表明方法准确、可靠,可用于后续分析.最后测得鞣花酸含量可达994 mg/kg.[结论]红树莓籽中鞣花酸的含量高于所报道的红树莓果实中鞣花酸的含量,可更进一步对其机理进行研究.