[Objective] To establish the method for determining puerarin and paeoniflorin in Yanbao I capsule. [Method] The HPLC analysis used chromatography glass column Diamonsil C18(2) (4.6 mm ×250 mm, 5 μm) with the detection wavelength at 250 nm for puerarin and 230 nm for paeoniflorin and the column temperature was set at 25 ℃. Following the injection of sample solution, an elution was initiated at a flow rate of 1 ml/min with anhydrous methanol-water ( V/V,28:72) for Puerarin and acetonitrile-0. 1% phosphoric acid ( V/V, 14:86) as mobile phase. [Result] The linear range of puerarin was from 0.001 18 to 0.007 08 μg/ml (r=0. 999 9). The average recovery of sample was 102.4% with RSD of 1. 17% (n=6). The linear range of paeoniflorin was from 0.044 to 0.261 μg/ml (r =0.999 9). The average recovery of sample was 97. 8% with RSD of 1.71 % (n=6). In Yanbao 1 capsule, the content of puerarin and paeoniflorin could reach 0. 055 and 0.168 mg/g, respectively. [ Conclusion] The method is simple, accurate and repeatable, which is suitable for determining puerarin and paeoniflorin in Yanbao 1 capsule.%[目的]建立眼保Ⅰ号胶囊中葛根素和芍药苷的含量洲定方法.[方法]色谱柱为Diamonsil C18(2)(4.6 mm ×250 mm,5 μm)；流速为1 ml/min;桂温为25℃；葛根素流动相为无水甲醇-水(28∶72,V/V),检测波长为250nm；芍药苷流动相为乙腈-浓度0.1％磷酸溶液(14∶86,V/V),检测波长为230 nm.[结果]葛根素在0.001 18-0.00708 μg/ml浓度范围内浓度与峰面积的线性关系良好(r=0.9999),平均回收率102.4％,RSD为1.17％(n=6)；芍药苷在0.044-0.261 μg/ml浓度范围内浓度与峰面积的线性关系良好(r=0.9999),平均回收率97.8％,RSD为1.71％(n=6)；眼保Ⅰ号胶囊内容物,葛根素和芍药苷的平均含量分别为0.055和0.168 mg/g.[结论]该方法简单,准确,重现性好,可用于眼保Ⅰ号胶囊中葛根素和芍药苷的含量检测.
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